Project:BB|3002

Title
Simplified and rapid molecular assays for diagnosis of Leishmaniasis and Human African Trypanosomiasis and parasite (sub-)species identification.
Acronym
 
URL
StartDate
2005-11-01
EndDate
2009-10-01
Amount
 

Abstract

The overall objective is to generate new knwoledge and technologies for improved diagnosis and control of Human African Trypanosomiasis (HAT) and Leishmaniasis (LEI).

The specific objectives are:
1.- To develop and validate low-tech molecular tests for laboratory diagnosis and (sub-) species differentiation in LEI and HAT.
2.- To develop and validate a sensitive point-of-care test for differential molecular LEI and HAT diagnosis based on ribosomal RNA (rRNA) detection.
3.- To strengthen research capacity of African investigators and research institutes on the biology and diagnosis of HAT and LEI.

Workpackages:
1. Project management and technology transfer
2. Parasite cryobank
3. Study of polymorphism within the diagnostic target sequences.
4. Standard protocols for sampling, preparation and storage bioclinical material.
5. Clinical sample cryobank.
6. Development and proof-of-principle of AMP-OC-DIAG/IDEN test
7. Laboratory evaluation (Phase II) of AMP-OC-DIAG/IDEN
8. Development and proof-of-principle of HYB-OC-DIAG
9. Laboratory (Phase II) evaluation of HYB-OC-DIAG
10. Field evaluation of HYB-OC-DIAG
11. Commercial evaluation of the developed tests (Phase IV).

Keywords

Leishmaniasis, Trypanosomiasis, Molecular diagnostic techniques

Fundings

Name Role Amount
unknown 2.390.000.00 EUR

People

Name Role Start End
Buscher, Philippe promotor 2005-11-01 2009-10-01

Orgunits

Name Role Start End
Parasite Diagnosis Unit member 2005-11-01 2009-10-01

created:2011-12-14 14:18:59 UTC, source:web

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