The general aim of our work is to evaluate the fumonisin production potential of the Fusarium species preserved in the collection. For this purpose we use two complementary approaches. The first one is the capillary electrophoresis technique forthe detection of fumonisin biosynthetic intermediates produced under different culture conditions. The second approach is based on a molecular screening of specific mRNAs related to key enzymes involved in the biosynthesis of this mycotoxin using the polymerase chain reaction technique. The combination of these two approaches should allow us to find out genes involved in the fumonisin biosynthesis pathway. Understanding the molecular biology and the biochemistry aspects of fumonisin production should help for the development of tools for quality assurance and mycotoxin risk assessment.