Vaccination and the control of abomasal nematodoses in ruminants.


The objective of the project is to assess the ability of selected native and recombinant helminth proteases to protect sheep and cattle against the co-infecting nematode species Haemonchun contortus and Teladorsagia cirsumcincta in sheep and Ostertagia ostertagi in cattle, which are known to express homologous enzymes. In addition, the humoral and cellular immune responses stimulated by vaccination will be evaluated.


ruminants, proteases, nematodes, vaccination


Name Role Start End
Vercruysse, Jozef promotor
Claerebout, Edwin co-promotor


Name Role Start End
Laboratory of Parasitology leader


Reference Role
Geldhof, P., Claerebout, E., Knox, D.P., Vercauteren, I., Loosova, A., Vercruysse, J., 2002. Vaccination of calves against Ostertagia ostertagi with cysteine proteinase enriched protein fractions. Parasite Immun 24, 263-270. author
De Maere, V., Vercauteren, I., Saverwyns, H., Claerebout, E., Berx, G., Vercruysse, J., 2002. Identification of potential protective antigens of Ostertagia ostertagi with local antibody probes. Parasitology 125, 383-391. author
Claerebout, E., Knox, D.P., Vercruysse, J., 2003. Current research and future prospects in the development of vaccines against gastrointestinal nematodes in cattle. Expert Rev Vaccines 2, 147-157. author
Meyvis Y., Callewaert N., Gevaert K., Timmerman E., Van Durme J., Schymkowitz J., Rousseau F., Vercruysse J., Claerebout E., Geldhof P., 2008. Hybrid N-glycans on the host protective activation-associated secreted proteins of Ostertagia ostertagi and their importance in immunogenicity. Mol Biochem Parasitol 161, 67-71. author
Visser A., Van Zeveren A.M., Meyvis Y., Peelaers I., Van den Broeck W., Gevaert K., Vercruysse J., Claerebout E., Geldhof P., 2008. Gender-enriched transcription of activation associated secreted proteins in Ostertagia ostertagi. Int J Parasitol 38, 455-465 author
Geldhof P., Meyvis Y., Vercruysse J., Claerebout E. 2008. Vaccine testing of a recombinant activation-associated secreted protein (ASP1) from Ostertagia ostertagi. Parasite Immunol 30, 57-60 author
Geldhof P., De Maere V., Vercruysse J., Claerebout E., 2007. Recombinant expression systems: the obstacle to helminth vaccines? Trends Parasitol 23, 527-532. author
Vercruysse J., Schetters T.P.M., Knox D.P., Willadsen P., Claerebout E., 2007. Control of parasitic disease using vaccines: an answer to drug resistance? Revue Scientifique et Technique-Office International des Epizooties 26, 105-115 author
Saverwyns, H., Visser, A., Nisbet, A., Peelaers, I., Gevaert, K., Vercruysse, J., Claerebout, E. and Geldhof, P., 2007. Identification and characterization of a novel specific secreted protein family for selected members of the subfamily Ostertagiinae (Nematoda). Parasitology135, 63-70 author
Meyvis, Y., Geldhof, P., Gevaert K., Timmerman, E., Vercruysse, J., Claerebout, E., 2007. Vaccination against Ostertagia ostertagi with subfractions of the protective ES-thiol fraction. Vet Parasitol 149, 239-345. author
Knox, D.P., Geldhof, P., Visser, A., Britton, C., 2007. RNA interference in parasitic nematodes: A reality check? Trends Parasitol (In press). author
Geldhof, P., Visser, A., Clark, D., Saunders, G., Britton, C., Gilleard, J., Berriman, M., Knox, D., 2007. RNA interference in parasitic helminths: current situation, potential pitfalls and future prospects. Parasitology (in press). author
Vercauteren, I., De Maere, V., Vercruysse, J., Stevens, M., Gevaert, K., Claerebout, E., 2006. A small heat shock protein of Ostertagia ostertagi: stage- and tissue-specific expression, heat inducibility and protection trial. J Parasitol 92, 1244-1250. author
Visser, A., Geldhof, P., De Maere, V., Knox, D.P., Vercruysse, J., Claerebout, E., 2006. Efficacy and specificity of RNA interference in larval life stages of Ostertagia ostertagi. Parasitology 133, 777-783. author
Claerebout, E., Vercauteren, I., Geldhof, P., Olbrechts, A., Zarlenga, D.S., Goddeeris, B., Vercruysse, J., 2005. Cytokine responses in immunised and non-immunised calves after Ostertagia ostertagi infection. Parasite Immun 27, 325-331. author
De Maere, V., Vercauteren, I., Gevaert K., Vercruysse, J., Claerebout, E., 2005. An aspartyl protease inhibitor of Ostertagia ostertagi: Molecular cloning, analysis of stage and tissue specific expression and vaccine trial. Mol Biochem Parasitol 141, 81-88. author
Claerebout, E., Smith, W.D., Pettit, D., Geldhof, P., Raes, S., Geurden, T., Vercruysse, J., 2005. Protection studies with a globin-enriched protein fraction of Ostertagia ostertagi. Vet Parasitol 128, 299-307. author
De Maere, V., Vercauteren, I., Geldhof, P., Gevaert K., Vercruysse, J., Claerebout, E., 2005. Molecular analysis of astacin-like metalloproteases of Ostertagia ostertagi. Parasitology 130, 89-98. author
Geldhof, P., Vercauteren, I., Vercruysse, J., Knox, D.P., Van Den Broeck, W., Claerebout, E., 2004. Validation of the protective capacity of the Ostertagia ostertagi ES-thiol antigens with different adjuvantia. Parasite Immun 26, 37-43 author
Vercauteren, I., Geldhof, P., Vercruysse, J., Peelaers, I., Van Den Broeck, W., Gevaert, K., Claerebout, E., 2004. Vaccination with an Ostertagia polyprotein allergen protects calves against a homologous challenge infection. Infect Immun 72(5), 2995-3001 author
Claerebout, E., Vercruysse, J., 2003. Assessment of the efficacy of helminth vaccines. J Parasitol 89 (Suppl.), pp.S202-S209 author
Geldhof, P., Vercauteren, I., Gevaert K., Staes A., Knox, D.P., Vandekerckhove J., Vercruysse, J., Claerebout, E., 2003. Activation-associated secreted proteins are the most abundant proteins in a host protective fraction of Ostertagia ostertagi. Mol Biochem Parasitol 128, 111-114 author
Geldhof, P., Vercauteren, I., Knox, D.P., De Maere, V., Vanzeveren, A., Berx, G., Vercruysse, J., Claerebout, E., 2003. Protein disulphide isomerase of Ostertagia ostertagi : an excretory-secretory product of L4 and adult worms? Int J Parasitol 33, 129-136. author

created:2011-12-14 14:18:59 UTC, source:iweto

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