In this project, an ecologically integrated spatial partition of Flanders was designed at two hierarchical levels: ecodistricts and ecoregions. The partition was based on the following abiotical components (in hierarchical order): climatology, geology, relief, geomorphology, geohydrology, hydrology of surface water, soil. Ecodistricts were defined on the basis of their homogeneity for most of these components. For this purpose, several maps were put in overlay and diverse information sources were studied separately. On a higher hierarchical level, ecodistricts were grouped into ecoregions, based on the similarity of their geological and geomorphological properties. A total of 36 ecodistricts, grouped into 12 ecoregions resulted from the study. The theoretical background of the method, a comparison with partitions in neighbough countries and suggestions for the use of ecodistricts and ecoregions for policy and research purposes were included in the report, as well as an description of the abiotical characteristics of each ecodistrict, and overlays with other ecologically relevant variables and existing legal and policy-linked maps. In addition, a case-study of the application of ecodistricts in area-directed environmental policy was added, with sensitivity maps at 3 scale levels for eutrophication, acidification and dessication for all ecodistricts.